Regenerative Medicine and Stem Cell Therapy
1.Autograft: Derived from recipient’s own body. Usually derived from Adipose cells but can also come from bone marrow.
2.Allograft: Derived from another human and implanted and/or infused into another. Species to species
3.Xenograft: Different species
All stem cells have 2 minimum qualities:
1.Stems cells are renewable. They undergo mitosis and double approximately every 28 hours for up to 65 generations.
2.Stem cells have potency: Potency is defined as the ability to differentiate into any cell in the body
Umbilical Cord Stem Cells How They Work
Paracrine: A form of cell to cell communication in which the stem cell produces a signal to induce changes in a nearby cell, altering the behavior or differentiation of those cells. The paracrine factors are secreted in the immediate extracellular environment.
Pericyte: Contractile cells that wrap around the endothelial cells of capillaries and venules throughout the body.
Trophic: Secrete cytokines and growth factors to induce cellular proliferation and angiogenesis.
Anti-inflammatory: In musculoskeletal trauma, the body releases inflammatory molecules such as IL-1, IL-2, IL-12, TNF-alpha, and INF-gamma, all initiated by the T-1 helper cells.(Pro-inflammatory cells). MSC’s in response secrete an array of anti-inflammatory proteins and immunomodulatory cytokines including PGE-2, tissue growth factor beta-2 (TGF beta-2), hepatocyte growth factor (HGF), nitric oxide, IL-4, IL-6, IL-10 and many more, all initiated by the T-2 helper cells. (anti-inflammatory cells)
Immunomodulatory: Capable of modifying or regulating one or more immune functions. ie. T-1 to T-2 modulation in autoimmune disorders.
Majority of autoimmune disorders are pro-inflammatory (T-1 weighted) thus balancing of the T-1 to T-2 ratio is important
Approximately 20% are T-2 weighted with the same balancing effect
Anti-apoptotic: Apoptosis-Programmed cell death, literally cell suicide.
MSC’s have the ability to rescue apoptotic cells induced by trauma, exposure to hypoxia, chemicals/acidity, mechanical damage or radiation.
Antimicrobial: MSC’s native immune defense against microbial infections is caused by multiple polypeptides. LL37 being the most common and has been shown to be effective against acute, chronic, and systemic infections. MSC’s have also been shown to be effective against protozoa. (Lyme’s disease)
Stem cell Therapy Saftey
Mesenchymal stem cells produce huge quantities of bio-molecules, some of which are immunosuppressive; These cells put up a curtain of molecules around themselves that allows donor (allogenic) stem cells to be transplanted into a recipient, free from an immune response. (Immune privileged) While the body will naturally release TNF-a, IFN-y, IL-12 and T-1 helper cells to initiate an immune response from the allogenic stem cells, the MSC’s release IL-4, IL-10 and T-2 helper cells to effectively regulate the immune response.
Arnold Caplan, PhD. Case Western Reserve University. Experimental and Molecular Medicine (2013) 45
Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine
▪Because HUCT stem cells are less mature than other cells, the body’s immune system is unable to recognize them as foreign and therefore they are not rejected. We’ve treated thousands of patients with umbilical cord stem cells and there has never been a single instance of rejection (graft vs. host disease). HUCT stem cells also proliferate/differentiate more efficiently than “older” cells, such as those found in the bone marrow and therefore, they are considered to be more “potent”.
▪Journal of Translational Medicine 2007, Stem Cell Therapy for Autism.
▪Thomas E. Ichim, Fabio Solano, Eduardo Glenn, Frank Morales, Leonard Smith, George Zabrecky, and Neil H. Riordan
HUCT Stem Cell Advantages
- Anyone can be treated since MSC’s are immune system privileged. No HLA matching necessary
- Best anti-inflammatory activity, immunomodulatory capacity and ability to stimulate regeneration.
▪Can be administered multiple times in uniform dosages that contain high cell counts. (Dose, frequency, and mode)
▪Dosing: 2-10 M/Kg of body weight
▪Frequency: 2&2. Follow like any other treatment
▪Mode: Intra-articular, I.V., intrathecal, trigger point
▪HUCT stem cells contain an abundant supply of MSC’s.
▪No need to collect stem cells through invasive procedures such as liposuction or bone marrow collection.
▪Consistent quality and quantity regardless of age or health of the patient
▪HUCT MSC’s proliferate/differentiate more efficiently than other types of stem cells and are therefore considered to be more potent.
▪* Neil Riordan, Ph.D., Stem Cell Institute, Panama;
▪** Mehling, Quartararo, et al., Journal of Stem Cell Research, 2015, 5.8
- A search of www.clinicaltrials.gov website provides information on the more than 4,000 clinical trials being conducted worldwide
Tissue Bank Regulations
▪Allogenic stem cells are considered a tissue and regulated by the AATB.
▪All cells are rigorously tested for diseases, according to tissue bank regulations
▪No expanding of product
▪A stem cell can only be used for a homologous function of the cell. That simply means that a stem cell can only be used for a function that it normally performs in the body, ie. Formation of Tissue, bone, cartilage, blood vessel, nerve, and cell regeneration.
▪Autoimmune Disorders (122 known)
R.A. Lupus Discoid Lupus
Lupus nephritis Interstit. cystitis Crohn’s disease
Autism Cirrhosis Alopecia areata
Lichen Planus Psoriasis Scleroderma
Vitiligo Addison’s dx Grave’s dx
Sjogren’s Endometriosis Celiac dx
Ulcerative Colitis Blood Disorders CREST syndrome
Polymyositis Lyme’s dx Psoriatic Arthritis
Sarcoidosis Fibromyalgia Myasthenia Gravis
MS Restless Leg Many Eye Conditions
Meniere’s dx Kawasaki’s Dx Vasculitis
Chronic Fatigue Gastritis Raynaud’s Phenom.
Ankylosing Spondylitis Guillain-Barre Syndrome
And many many more
▪Chronic Joint Pain
▪Low Back Pain
▪Bulging or Herniated Discs
▪Spinal Cord Injuries
▪Meniscus, ACL or MCL tears
▪Lung Disease: COPD, Asthma, etc.
▪Diabetes Type II
▪Alcohol or Drug Addiction
▪ALS (Lou Gehrig’s dx)
▪Accelerates Healing/Reduces Post-Op Inflammation
▪Etc, etc, etc………
Basically, anything that the stem cell naturally does in the body. ie homologous function of the cell